ATHLETICS NEWS - A sample taken from Carter at the 2008 Games in Beijing was found last January to have contained a banned stimulant.
That meant the Jamaican 4x100m team had to forfeit gold.
The Court of Arbitration for Sport (Cas) ruled: "Reanalysis confirmed the presence of methylhexaneamine."
The Jamaican team featured Carter, Bolt, Michael Frater and Asafa Powell.
A judgement said: "We [do] not accept any of the arguments raised by Nesta Carter contending that the test results should be ignored or that the decision should otherwise be overturned for certain alleged failures.
"Accordingly, the Cas panel dismissed the appeal and the decision is confirmed."
Carter appealed against the decision last February and a hearing was held in Switzerland in November.