Bolt's 4x100m relay team-mate Nesta Carter is reported to have tested positive for a banned substance during retesting of 454 samples from the 2008 Beijing Olympics.
The result of a 'B' Sample follow-up test is not yet known.
"I'm not too pleased about the situation," said reigning World and Olympic sprint champion Bolt, 29.
Bolt was speaking after he recorded the second-fastest 100m time of the year, winning in 9.88 seconds in Jamaica.
"For years you've worked hard to accumulate gold medals and you work hard to be a champion, so it's heartbreaking, but it's one of those things," Bolt told RJR communications in Kingston.
