After Monday's rest day, the 100th edition of the race resumes Tuesday with a 181km fourth stage from Cefalu in the north of Sicily to the summit of Europe's highest active volcano.
Fernando Gaviria, another Colombian, who rides for Quick Step, will start in the pink jersey after he took the race lead from fellow sprinter Andre Greipel by winning stage three to Cagliari, Sardinia, on Sunday.
But after three days of seeing the fast men of the peloton sprinting for glory, fans get a chance to tune in for some early-race salvos in the definitive battle for the pink jersey.
It is the first summit finish of the race and Nibali, the winner in 2013 and 2016, expects 2014 champion Quintana to put the hammer down.
